Content:

• Introduction to disease concept

• How to optimize targeted therapies

• Phytochemistry and rare diseases’ therapy

• Plant extract preparation (p)

• Basics of cell culture from past to future

• Passaging and secondary culture (p)

• Cell culture handling and troubleshooting (p)

• MTT cell-based assay

• Passaging technique (p)

• Treatment preparation (p)

• Treatment administration (p)

• MTT induction (p)

• Cell viability data analysis (p)

• Mutation related to various diseases

• RNA extraction from cell line and primary tissues (p)

• Molecular biology of cell

• Central dogma

• RT-qPCR (p)

• Data analysis of RT-qPCR (p)