• Introduction to disease concept
• How to optimize targeted therapies
• Phytochemistry and rare diseases’ therapy
• Plant extract preparationÂ
• Basics of cell culture from past to future
• Passaging and secondary cultureÂ
• Cell culture handling and troubleshootingÂ
• MTT cell-based assay Passaging techniqueÂ
•Treatment preparationÂ
•Treatment administrationÂ
• MTT induction
• Cell viability data analysisÂ
• Mutation related to various diseases
• RNA extraction from cell line and primary tissuesÂ
• Molecular biology of cell
• Central dogma
• RT-qPCRÂ
• Data analysis of RT-qPCRÂ